Antimicrobial Peptide Genes Present in Indigenous Isolates of Bacillus spp. Exhibiting Antimicrobical Properties

Abstract

An attempt was made to detect the presence of antimicrobial peptide genes (AMPs) in ten different indigenous isolates of Bacillus spp. proved to be effective against M. incognita, Fusarium oxysporum f.sp.lycopersici and their disease complex. The study revealed the presence of iturin A, iturin C, iturin D, surfactin, bacilomycin D, fengycin D, zwitermycin, bacillomycin bac D, bacilysin bcc AB and fengycin CAE in the above isolates of Bacillus spp. Among the ten different AMPs, the iturin A gene was found to present in all the ten isolates of Bacillus spp. Of all indigenous isolates B. thuringiensis (TLBRE2), B. cereus (CLB2D) were in possession of higher number of eight AMPs. It was followed by B. tequilensis (TLB2) with seven genes, B. licheniformis and B. Cereus (TSB 3, CLB2 and TSB4D) with six genes, B. weihenstephanensis (CLB3) and B. subtilis (TLBRE1) with four genes and B. subtilis (TSB5) with three genes.