Prevalensi dan Pola Sensitivitas Antimikroba Multidrug Resistant Pseudomonas aeruginosa di RSUD Arifin Achmad

Abstract

Antibacterial Evaluation of Extract Beehive (Apis dorsata) against Growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Bacterial infectious diseases are serious health problems. A natural antibacterial that can be used to inhibit bacterial growth of honeycomb (propolis, honey bag, egg bag and pollen bag) containing secondary metabolite compounds such as flavonoids, tannins and phenolic acids. The purpose of this research is to know the antibacterial activity of honeycomb extract from each solvent used and to know the effect of honeycomb extract concentration against the bacteria growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Antibacterial testing was performed by the method of paper disc diffusion with soaking time 1 hour followed by incubation for 3 x 24 hours. The results show that antibacterial activity of methanol honeycomb extract had the highest activity in E. coli bacteria of 3.8 mm at 8% concentration, followed by those of ethyl acetate honeycomb extract and n-hexan honeycomb extract which have antibacterial activity of 3.72 mm and 16.1 mm at 8% concentration against S. aureus and E. coli, respectively. The effect of extract concentration is the higher the concentration the greater the inhibitory power of extract against the bacteria growth of S. aureus, E. coli and P. aeruginosa.