Prolylendopeptidase inhibitory activity of a glial fibrillary acidic protein fragment and other proline-rich peptides

Abstract

The brain prolylendopeptidase (PEP) inhibitory activity of synthetic peptides related to the bovine brain-derived PEP inhibitor, GFAP-(38–55) (MPPPLPARVDFSLAGALN), was investigated. Homologous peptides such as MPPPLPTRVDFSLAGALN (human type) and MTPPLPARVDFSLAGALN (mouse type) also inhibited PEP. Among various synthetic fragments of GFAP-(38–55), only MPPPLP had a Ki essentially the same as that of GFAP-(38–55). Similar synthetic proline-rich peptides such as synapsin fragments and SH3 domain-binding peptides had more or less inhibitory activity. © 1996, Taylor & Francis Group, LLC. All rights reserved.