Long non‑coding RNA B4GALT1‑Antisense RNA 1/microRNA‑30e/SRY‑box transcription factor 9 signaling axis contributes to non‑small cell lung cancer cell growth

Abstract

Long non‑coding (lnc) RNAs serve crucial func‑ tions in human cancers. However, the involvement of the lncRNA B4GALT1‑antisense RNA 1 (AS1) in non‑small cell lung cancer (NSCLC) has not been extensively studied. Reverse transcription‑quantitative PCR was performed to detect B4GALT1‑AS1 levels in NSCLC tissues and cell lines. Potential influences of B4GALT1‑AS1 on biological func‑ tions of NSCLC were assessed through a series of in vitro experiments, and the molecular mechanism was determined via RNA immunoprecipitation (RIP) and bioinformatics analyses. The results of the present study demonstrated that knockdown of B4GALT1‑AS1 significantly attenuated the proliferative ability and clonality of H1299 and A549 cells. In the present study, B4GALT1‑AS1 competed as an endogenous RNA by sequestering microRNA‑30e (miR‑30e) leading to an enhanced expression of SRY‑box transcription factor 9 (SOX9). The effects of silencing B4GALT1‑AS1 on NSCLC cells proliferation could be ameliorated by inhibiting miR‑30e or restoring SOX9. Hence, B4GALT1‑AS1 acted as a lncRNA that drives tumor progression in NSCLC via the regulation of the miR‑30e/SOX9 axis. The findings of the present study indicated that the B4GALT1‑AS1/miR‑30e/SOX9 axis maybe an effective target for NSCLC treatment and management.