Fatty acids ameliorate doxorubicin-induced intracellular Ca2+ increase and apoptosis in rat cardiomyocytes

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Abstract

Doxorubicin (Dox) is a highly effective anticancer drug but exhibits cumulative dose-dependent cardiomyopathy. In this study, we investigated effects of Magnolia seed extract (MagS) on the Dox-induced cardiotoxicity. The results showed that MagS significantly reduces doxorubicin (Dox)-induced increase in intracellular Ca2+ concentration ([Ca2+]i), generation of reactive oxygen species (ROS), and apoptosis in rat cardiomyocytes. Analyses of the bioactive compounds in MagS by thin layer chromatography and gas chromatography/mass spectroscopy revealed that bioactive compounds in MagS are linoleic acid, oleic acid, and palmitic acid. All three fatty acids were able to inhibit the Dox-induced increase in [Ca 2+]i, ROS generation, and apoptosis with a similar potency. Efficacy of MagS was examined in in vivo using a murine Dox-induced cardiomyopathy model. Dox (12 mg/kg, intravenously) was administered to mice and treated with the MagS (2 mg/kg/d, intraperitoneally) or saline for three weeks. Dox-treated mice showed structural disarray in heart tissue, including lymphocyte infiltration and loss of body weight. In contrast, treatment of the MagS substantially attenuated the Dox-induced cardiac damages including the loss of body weight. These results indicate that fatty acids in MagS and other seeds may ameliorate cardiotoxicity of the anticancer drug. © 2008 Pharmaceutical Society of Japan.

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Park, K. H., Kim, S. Y., Gul, R., Kim, B. J., Jang, K. Y., Chung, H. T., & Sohn, D. H. (2008). Fatty acids ameliorate doxorubicin-induced intracellular Ca2+ increase and apoptosis in rat cardiomyocytes. Biological and Pharmaceutical Bulletin, 31(5), 809–815. https://doi.org/10.1248/bpb.31.809

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