Bioremediation of petroleum—polluted soils by adsorptive immobilized corynebacterium sp. rb—96

Abstract

Living cells of oil-degrading bacteria, Corynebacterium sp. RB—96, were immobilized on two different types of zeolite materials (rhodopin and rhodosorb, “Ecotech”, Sofia) by adsorption. The optimal parameters of immobilization (concentration of the carrier, time for adsorption, growth phase of the bacteria) were investigated. The biodegradation of crude oil of light and heavy type by cells immobilized on these zeolites was tested. The cells immobilized on rhodopin were better degraders then the free cells. The cells attached to rhodosorb degraded crude oil more slowly in comparison with the immobilized cells. Corynebacterium sp. RB—96 (free and adsorbed) oxidized light crude oil with preference. © 2000 Taylor and Francis Group, LLC.