Assays of angiogenesis in vitro provide insights into vascular development and are useful in studies of agents that modulate blood vessel formation. This chapter describes techniques to induce angiogenesis-like sprouting from aortic and microvascular explants cultured in 3-dimensional native collagen gels. The chapter focuses on explants derived from mice and use of a miniaturized format that permits efficient utilization of reagents, ease of processing, rapid analysis, and conventional imaging. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Reed, M. J., & Vernon, R. B. (2012). Miniaturized assays of angiogenesis in vitro. Methods in Molecular Biology, 843, 87–98. https://doi.org/10.1007/978-1-61779-523-7_9
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