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Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns

  • Kwon C
  • Kim Y
  • Jeon H
Journal of Functional Biomaterials (2017) 8(3) 34
DOI: 10.3390/jfb8030034
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Abstract

Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed. Microgroove patterns were variable, with widths of 3, 5, and 10 µm. After the seeding, time-lapse images were taken under controlled cell culturing conditions. Cell sheet borders were drawn in order to assess collective migration rate. Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells.

Figures

  • Figure 1. Schematic illustration of the fabrication process of patterned polydimethylsiloxane (PDMS). The master pattern of PDMS mold process using SU-8 photoresist and subsequent generation of the PDMS replication stamp.
  • Figure 2. (a) Cell seeding procedure. PDMS block is covered on the patterned surface to prevent from the cell seeding point. (b) After PDMS block is removed, the cells are placed on the pattern for 15 min for cell adhesion. (c) The cell migration rates were measured from starting point every 24 h.
  • Figure 3. (a) Scanning electronic microscopy and (b) Immunofluorescence images of B-3 on various surfaces. Cells on r3g5, r5g5, r5g3. r5g10, r10g5, and non-patterned surface acted as control. Blue, Green, and Red fluorescence resents nucleus, F-actin, and vi culin, respectively. S ale bars indicate (a) 50 µm and (b) 20 µm.
  • Figure 4. Elongation index. Elongation parameter is evaluated by dividing ce l lengt i t . The different values indicate circular or linear cell shape (n = 10). Data expressed as mean ±SD. p < 0.001 versus control.
  • Figure 5. Migration rates of B-3 for 4 days. (a) The cell migrations at different days are traced. Control group of the cell migration rate (left) and r3g5 group (right) from day 0 to day 4 and the cell migrations Figure 5. Migrat on rates of B-3 for 4 days. (a ce l migrations at different days r traced. Control group of the cell migration rate (left) and r3g5 group (right) from day 0 to day 4 and the cell migrations on day 2 (bottom), respectively. (b) The cell migration rates of B-3 on different types of pattern. Scale bars indicate 100 µm. Data represent the mean ±SD of three independent experiments. p < 0.001 versus control.
  • Figure 6. Cell proliferation rates of different surfaces on day 1 (white), day 3 (light gray), and day 5 (black) after the seeding. The absorbance was read at 450 nm by a spectrophotometer microplate reader. Data represent the mean ±SD of three independent experiments. p < 0.001 versus control.

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APA

Kwon, C., Kim, Y., & Jeon, H. (2017). Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns. Journal of Functional Biomaterials, 8(3), 34. https://doi.org/10.3390/jfb8030034

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