Prevalencia de infección por el virus del Nilo occidental en dos zoológicos del estado de Tabasco

Abstract

Objective. To determine the prevalence of West Nile Virus (WNV) infection in animals, mosquitoes and employees from two zoos of Tabasco state, Mexico. Material and Methods. WNV antibodies were detected by blocking ELISA in serum samples from animals. Viral RNA was detected by RT-PCR from mosquitoes and serum samples from employees at "Yum-Ká" zoo. Results. Seroprevalence in birds was 25.65% (19/74) and 85% (6/7) in reptiles from "La Venta" zoo. Thirty-one percent of birds (50/160) and 34.48% mammals (16/29) at the "Yum-Ká" zoo, were seropositive. All human serum samples from Yum-ká zoo were negative by RT-PCR. A pool of mosquitoes (Culex quinquefasciatus) was positive for WNV. Conclusions. The presence of WNV antibodies in animals from both zoos and the detection of viral genome in mosquitoes demonstrate the presence of WNV in this region and indicates a potential risk of infection in animals and humans.