Abstract
Intravital multiphoton microscopy (MP-IVM) is a powerful imaging approach that allows direct visualization of cells within their native environment in real time. Multiphoton imaging of immune cells has been performed in different tissues, and these studies have revealed intriguing insights into the unique migratory and interactive behavior of immune cells in the steady-state and during disease conditions. Here we provide an overview of a MP-IVM model of the mouse ear skin, as well as the benefits, limitations and pitfalls of this approach. We also discuss the prospects of intravital imaging in the areas of image analysis, data management and standardization. © 2012 Springer Berlin Heidelberg.
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CITATION STYLE
Keeble, J., Goh, C. C., Wang, Y., Weninger, W., & Ng, L. G. (2012). Intravital multiphoton imaging of immune cells. In Advances in Intelligent and Soft Computing (Vol. 120, pp. 3–16). https://doi.org/10.1007/978-3-642-25547-2_1
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