The polymorphism of prolactin gene in native chicken Zabol region

Abstract

The aim of this study was to determine genetic polymorphism of prolactin gene for native chickens Zabol. Avian prolactin hormone located in chromosome No: 2. It is a polypeptide that it molecular weight is 21700-26000 Dalton and effective in increasing broodiness and commonly results in regression of the ovary. In this study, blood samples were collected from 30 native chickens randomly and DNA was extracted. The PCR was used to amplify 439 bp fragments for prolactin gene locus. The amplified fragment was digested with Alu I enzyme. Allele frequencies of T and C prolactin hormone gene for native chickens were 0/67, 0/33, respectively. Genotype frequencies TT, CT, CC in herd were 53/33, 26/67 and 20/00%, respectively. The results show that PCR-RFLP method is proper for genotype determination of prolactin gene chicken studies. © Medwell Journals, 2011.