In vivo and in vitro variation in protein profiling in withania somnifera (L.) dunal

Abstract

Proteomics is a leading new technology for the high-throughput analysis of proteins on a genome-wide scale in living system. This technique was applied to investigate the protein changes under in vitro and in vivo conditions, since in vitro cultures is considered to be an alternative approach to traditional agriculture in the industrial production of the biomolecules. The overall goal of this project was to investigate the changes in protein expression under in vitro and in vivo leaves tissues of Withania somnifera. Of ̃28 protein bands resolved in the two-dimensional gels, 21 protein bands were similarly expressed in both in vitro and in vivo root tissues 1 protein in in vivo condition and 2 protein bands were differentially expressed only in in vitro tissue. This is the first report on the comparison of in vitro and in vivo samples by establishment of a 2-D reference proteome map of Withania somnifera ©2014 Academic Journals Inc.