Association of Sirtuin 1 Gene Polymorphisms with the Risk of Coronary Heart Disease in Chinese Han Patients with Type 2 Diabetes Mellitus

Abstract

Aims. To explore the associations between polymorphisms in SIRT1 and coronary heart disease (CHD) risk in Chinese Han patients with type 2 diabetes (T2D). Methods. This case-controlled study enrolled 492 patients with T2D: 297 with CHD and 195 without CHD. Five SIRT1 haplotype-tagging single-nucleotide polymorphisms (rs3818291, rs12242965, rs3818292, rs4746720, and rs16924934) were selected from Chinese Han data in the GRCh37.p13 phase 3 database and genotyped by polymerase chain reaction-restriction fraction length polymorphism or sequencing. Results. The rs16924934 G allele was associated with a higher risk of CHD than the A allele (odds ratio OR=1.429; 95% confidence interval CI=1.003-2.037; P=0.048). Using an additive inheritance model, the rs3818291 G/A genotype was associated with a higher CHD risk than the G/G genotype (OR'=1.683; 95%CI=1.033-2.743; P'=0.037 after adjustment for CHD risk factors). Smokers carrying G/A or A/A rs3818291 genotypes had a 3-fold higher CHD risk than those carrying GG (adjusted OR'=3.035; P'=0.011) and a 2.6-fold higher CHD risk than nonsmokers carrying GG (adjusted OR'=2.604; P'=0.033). Conclusions. Genetic polymorphisms of SIRT1 are associated with the risk of CHD in a Chinese Han population with T2D.