The effectiveness of potential marine plants as pathogen bacterial inhibitory compaunds

Abstract

With the development of research and technology, marine plants can be a bio-prospective for secondary metabolites product. The content of marine plant as a source of secondary metabolites product has an important role as a source of antibacterial pathogen compounds. The study aimed to utilize extracts of Ipomoea pes-caprae, Sonneratia alba, Gracilaria debilis and Dictyoshaeria versluysii on the antibacterial formation of pathogenic bacteria. The research was conducted in laboratory following several steps procedure i.e. extraction of samples with ethyl acetate and alcohol as solvent and then diffusely testing of antibacterial activity. All experiment was done with two replications. Pathogen bacteria used in this experiment are Escherichia coli, Salmonella typhimurium, Enterococci, Staphylococcus aureus, Clostridium perfringens, and Pseudomonas. The result showed that extract ethyl acetate performs better than extract of alcohol to inhibit pathogen bacteria. The extract of ethyl acetate created inhibitory zone activity against E. coli was 2.9 ± 0.20 mm (I. pes-caprae), 2.8 ± 0.12 mm (Sonneratia alba), 26.0 ± 0.16 mm (G. debilis), and 32.0 ± 0.08 mm (D. versluysii). Result of inhibitory zone activity against Salmonella were 30.5 ± 0.10 mm (I. pes-caprae), 2.5 ± 0.10 mm (S. alba), 27.5 ± 0.16 mm (G. debilis), and 29.18 ± 0.18 mm (D. versluysii). Inhibitory activity zone for Enterococci were 2.5 ± 0.10 mm (I. pes-caprae), 3.4 ± 0.30 mm (S. alba), 38.0 ± 0.10 mm (G. debilis), and 42.0 ± 0.18 mm (D. versluysii), whilst for S. aureus the inhibitory activity zone were 2.5 ± 0.1 mm, 3.4 ± 0.10 mm, 30.0 ± 0.12 mm, and 31.0 ± 0.1 mm for I. pescaprae, S. alba,, G. debilis and D. versluysii respectively. Results of inhibitory activity zone for C. perfringens were 2.9 ± 0.2 mm, 2.7 ± 0.12 mm, 26.5 ± 0.14 mm and 32.0 ± 0.1 mm for I. pes-caprae, S. alba, G. debilis and D. versluysii while for Pseudomonas were 2.7 ± 0.2 mm, 2.4 ±0.14 mm, 28.0 ± 0.10 mm and 26.0 ± 0.22 mm.