Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system

Abstract

Infectious disease-related illnesses are a significant threat to human health resulting in substantial morbidity and mortality, worldwide. Timely and accurate diagnostic tools are critical for patient treatment decisions and disease outcomes. Molecular diagnostics are revolutionizing the clinical practice of infectious disease. The various formats of nucleic acid amplification are the most frequently used molecular tests in the diagnosis of infectious diseases due to its exquisite sensitivity and specificity. Gel electrophoresis and Southern hybridization are two basic technologies that are used to display the specific amplification of targeted gene and are still used in the laboratories for diagnosis because it is such a powerful technique, and yet reasonably easy and inexpensive.