Objective: Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. Results: This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni2+ affinity column and eluted at 100-150 mM imidazole, has potential as a recombinant antigen for further study.
CITATION STYLE
Cartwright, S. P., Bill, R. M., Sy, B. T., Tran-Van, H., & Nguyen, H. M. (2017). Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris. BMC Research Notes, 10(1). https://doi.org/10.1186/s13104-017-2692-8
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