Confocal microscopy, which is a major advance upon normal light microscopy, has been used in a number of scientific fields. By confocal microscopy techniques, cells and tissues can be visualized deeply, and three-dimensional images created. Compared with conventional microscopes, confocal microscope improves the resolution of images by eliminating out-of-focus light. Moreover, confocal microscope has a higher level of sensitivity due to highly sensitive light detectors and the ability to accumulate images captured over time. In present studies, a series of Weeping Forsythia pollen digital images (35 images in total) were acquired with confocal microscope, and the three-dimensional digital image of the pollen reconstructed with confocal microscope. Our results indicate that it's a very easy job to analysis three-dimensional digital image of the pollen with confocal microscope and the probe Acridine orange (AO). © 2009 Springer Science+Business Media, LLC.
CITATION STYLE
Liu, D., Chen, Z., Xu, H., Liu, W., & Wang, L. (2009). Reconstruction of 3D digital image of Weeping Forsythia pollen. In IFIP International Federation for Information Processing (Vol. 293, pp. 329–334). Springer Science and Business Media, LLC. https://doi.org/10.1007/978-1-4419-0209-2_35
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