Phosphorylation of nucleosides and nucleotides with inorganic monoimido-cyclo-triphosphate

Abstract

The phosphorylation of nucleosides (adenosine, guanosine, cytidine, and uridine) and nucleotides (adenosine 5′-monophosphate, guanosine 5′-monophosphate, cytidine 5′-monophosphate and uridine 5′-monophosphate) has been achieved using inorganic monoimido-cyclo- triphosphate (MCTP, Na3P3O8NH) in aqueous solution. In this reaction, the 2′-OH or 3′-OH group of the β-D-ribofuranose unit was phosphorylated and the total yield was more than 30% and 14%, respectively. The main products were 2′- diphosphoramidophosphononucleoside and 2′- diphosphoramidophosphononucleoside 5′-monophosphate. © 2008 Pharmaceutical Society of Japan.