RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.
CITATION STYLE
Shibata, T., Suzuki, Y., Sugiyama, H., Endo, M., & Saito, H. (2015). Folding RNA–protein complex into designed nanostructures. Methods in Molecular Biology, 1316, 169–179. https://doi.org/10.1007/978-1-4939-2730-2_14
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