Summary: Transient expression is the nonstable expression of a transgene whereby the transgene does not integrate into the host's genomic DNA. Transient expression assays have 20 years of history in plant molecular biology research, being used to answer a variety of questions. The method described here allows the ability to test promoter activity for seed-specific expression by quantifying reporter protein production in immature seed tissues. This method is especially suited to test vector activity for stable expression, test promoter activity, and discern regions of a promoter that is necessary for transcription in seed tissues. The transient expression assay is a tool that has aided a great deal of molecular biology research. © 2009 Humana Press.
CITATION STYLE
Shepherd, C. T. (2009). Transient expression of GFP in immature seed tissues. Methods in Molecular Biology, 526, 23–28. https://doi.org/10.1007/978-1-59745-494-0_2
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