Ligand Engineering via Yeast Surface Display and Adherent Cell Panning

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Abstract

High-throughput ligand discovery and evolution—via genotype-phenotype linkage strategies—empower molecularly targeted therapy, diagnostics, and fundamental science. Maintaining high-quality target antigen in these selections, particularly for membrane targets, is often a technical challenge. Panning yeast-displayed ligand libraries on intact mammalian cells expressing the molecular target has emerged as an effective strategy. Herein we describe the techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.

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Stern, L. A., Lown, P. S., & Hackel, B. J. (2020). Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. In Methods in Molecular Biology (Vol. 2070, pp. 303–320). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9853-1_17

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