Antitumor effects and mechanisms of pyropheophorbide‑α methyl ester‑mediated photodynamic therapy on the human osteosarcoma cell line MG‑63

Abstract

Photodynamic therapy (PDT) is a promising treatment for osteosarcoma, and pyropheophorbide-α methyl ester (MPPa) is a second-generation photosensitizer for tumor treatment. The present study aimed to determine the efficacy and possible mechanisms of MPPa-PDT in the treatment of osteosarcoma MG-63 cells. Flow cytometry and western blotting were used to detect cell cycle-related indicators Cyclin D1, Cyclin E, Cyclin A and Cyclin B1. Cell migration and invasion abilities were detected using wound-healing and Transwell chamber assays. Cellular endoplasmic reticulum stress (ERS), autophagy and apoptosis-related indicators were detected by flow cytometry and western blotting. The results demonstrated that MPPa-PDT blocked the MG-63 cell cycle and inhibited cell migration and invasion. Additionally, MPPa-PDT inhibited the activation of the Akt/mammalian target of rapamycin (mTOR) pathway. MG-63 cells underwent ERS-induced apoptosis following MPPa-PDT treatment. Pretreatment with the mTOR phosphorylation inhibitor rapamycin affected the autophagy of MPPa-PDT-induced osteosarcoma MG-63 cells and enhanced apoptosis through targeting mTOR.