Preparing Tissues for IHC Staining

Abstract

Fresh histological tissues Tissues cannot be used ``as is'' and must undergo special processing to turn them into a suitable experimental material: tissues need to be first chemically fixed and then cut into thin sections using special histological instruments. Fixation and tissue sectioning Tissue section are critical to the success of IHC staining. If they are not done correctly, staining quality can be badly compromised regardless of the use of high-quality primary and secondary antibodiesAntibodies secondary, buffersBuffers, and detection reagents.