Cell lineage analyses in living tissues

Abstract

Developmental biologists require methods for marking cell lineages as they arise in living tissues. Traditionally, lineages have been traced in fi xed tissues but these observations are dif fi cult to verify. We present a method by which a progenitor cell and all of its lineage become marked by a nuclear-localised fl uorescent protein. This allows rapid estimation of the effects of genetic or physical manipulation of developing tissues. Heat shock is used to activate YFP expression in single progenitor cells which is heritable by all daughter cells in subsequent rounds of mitosis. Heat shock can be applied to specimens generally using an incubator to generate random lineage patterns or more speci fi cally to single cells or small regions using laser activation of the lineage marking system. © Springer Science+Business Media New York 2013.