Background: A blunted muscle protein synthetic response to protein ingestion may contribute to the age related loss of muscle tissue. We hypothesized that the greater endogenous insulin release following co-ingestion of carbohydrate facilitates post-prandial muscle protein accretion after ingesting a meal-like bolus of protein in older males. Methods. Twenty-four healthy older men (75±1 y) were randomly assigned to ingest 20 g intrinsically L-[1-§ssup§13§esup§C] phenylalanine-labeled casein protein with (PRO-CHO) or without (PRO) 40 g carbohydrate. Ingestion of specifically produced intrinsically L-[1-§ssup§13§esup§C] phenylalanine labeled protein allowed us to assess post-prandial incorporation of dietary protein derived amino acids into muscle protein. Blood samples were collected at regular intervals, with muscle biopsies being obtained prior to and 2 and 6 h after protein ingestion. Results: Plasma glucose and insulin concentrations showed a greater increase in PRO-CHO compared with PRO (P<0.001). Muscle protein-bound L-[1-§ssup§13§esup§C] phenylalanine enrichments tended to increase to a greater extent in PRO-CHO compared with PRO during the first 2 h after protein ingestion (0.0072±0.0013 vs 0.0046±0.010 MPE, respectively; P=0.13). However, 6 h after protein ingestion, differences in muscle protein-bound L-[1-§ssup§13§esup§C] phenylalanine enrichments were no longer observed between experiments (0.0213±0.0024 vs 0.0185±0.0010 MPE, respectively; P=0.30). Conclusions: This study shows that carbohydrate ingestion may accelerate, but does not further augment post-prandial incorporation of dietary protein derived amino acids into muscle protein in healthy elderly men. © 2013 Hamer et al; licensee BioMed Central Ltd.
CITATION STYLE
Hamer, H. M., Wall, B. T., Kiskini, A., De Lange, A., Groen, B. B. L., Bakker, J. A., … Van Loon, L. J. C. (2013). Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men. Nutrition and Metabolism, 10(1). https://doi.org/10.1186/1743-7075-10-15
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