Stem cell in vitro culture is a useful model system to study mechanisms underlying transitions between defined cell states. Epiblast stem cells, in addition to being capable of somatic differentiation, can be converted to a more primitive embryonic stem cell-like state, by overexpression of specific transcription factors. Here, we describe a reliable method to accomplish—and potentially further study—the transgene-independent reversion from epiblast stem cells to ES cells using administration of specific growth factors and small molecule inhibitors.
CITATION STYLE
Rao, J., & Greber, B. (2014). Conversion of epiblast stem cells to embryonic stem cells using growth factors and small molecule inhibitors. Methods in Molecular Biology, 1150, 215–226. https://doi.org/10.1007/978-1-4939-0512-6_14
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