Biocompatibilidad del fosfato tricálcico con quitosano para uso en regeneración ósea / Biocompatibility of tricalcium phosphate with chitosan for bone regeneration purposes

Abstract

Background: It is necessary to develop bone grafts capable to regenerate critical size bone defects. Objective: To evaluate the biocompatibility in vivo of tricalcium phosphate with chitosan (TPC) in skin, muscle, and bone. Methods: 15 Wistar rats were assigned to groups A (skin), B (muscle), C, D, and E (bone). TPC was placed in each tissue. In groups C-E, critical size bone defect was grafted with TPC and collagen sponge was placed adjacent to test sites as a control. Animals from groups A and B were sacrificed after 20 days, while groups C-E at days 45, 60, and 80. Inflammatory response was evaluated in all tissues. To assess biocompatibility, the percentage of cells was evaluated as none (0%), low (? 30%), moderate (30-50%), and high (?50%). Results: There were no signals of ulceration or suppuration in skin, muscle, and bone. After 80 days, TPC was incorporated into a fibrotic structure and osseointegrated to native bone. Conclusion: TPC was biocompatible with skin, muscle, and bone.