Determination of the optimum ratio of spermatozoa per oocyte of the piabanha Brycon insignis

Abstract

The optimum spermatozoa:oocyte ratio of piabanha Brycon insignis was studied. Two males and two females were induced to spawn, and the gametes were stripped after 200 hourgrades starting from the application of carp pituitary gland. Oocytes from two females were mixed, and samples of 2g (701 oocytes/g) were collected from the obtained pool and placed into plastic cups. The semen from the two males, after mixed to compose a pool, was diluted in NaCl solution (1.2%) so that, after the addition of 1ml of diluted sperm into the oocytes, the following spermatozoa:oocyte ratios (tri-replicated) were obtained: T1=86,662, T2=173,324, T3=259,986, T4=346,648 and T5=433,310. After the activation using 1% NaHCO3 and fertilization, the eggs were transferred to incubators, and observed the percentages of fertilization relative to the control. T1=35.7%, T2=53.1%, T3=79.1%, T4=93.4% and T5=87.8%. Undiluted semen was used as quot;controlquot;, which showed 65% of fertilization. The percentage of fertilization (relative to the control) increased linearly according to the regression Ŷ= 15.55 + 0.0002297X (P<0.01; R2=0.98), until the proportion of 314,481 spermatozoa per oocyte, and, from this point, the fertilization rate maintained at 88%.