Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.
CITATION STYLE
Pisharath, H., & Parsons, M. J. (2009). Nitroreductase-mediated cell ablation in transgenic zebrafish embryos. Methods in Molecular Biology (Clifton, N.J.), 546, 133–143. https://doi.org/10.1007/978-1-60327-977-2_9
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