Strong cation exchange chromatography in analysis of posttranslational modifications: Innovations and perspectives

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Abstract

Strong cation exchange (SCX) chromatography has been utilized as an excellent separation technique that can be combined with reversed-phase (RP) chromatography, which is frequently used in peptide mass spectrometry. Although SCX is valuable as the second component of such two-dimensional separation methods, its application goes far beyond efficient fractionation of complex peptide mixtures. Here I describe how SCX facilitates mapping of the protein posttranslational modifications (PTMs), specifically phosphorylation and N-terminal acetylation. The SCX chromatography has been mainly used for enrichment of these two PTMs, but it might also be beneficial for high-throughput analysis of other modifications that alter the net charge of a peptide. © 2011 Mariola J. Edelmann.

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Edelmann, M. J. (2011). Strong cation exchange chromatography in analysis of posttranslational modifications: Innovations and perspectives. Journal of Biomedicine and Biotechnology. https://doi.org/10.1155/2011/936508

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