Notoacmeibacter ruber sp. nov., a novel endophytic bacterium isolated from leaf of Rhizophora stylosa

Abstract

A Gram-stain negative, aerobic, coccus-shaped bacterium, designated strain M2BS9Y-3-1 T , was isolated from a surface-sterilized leaf of Rhizophora stylosa. It grew in the pH range 5.0–9.0 (optimum, 7.0) and at temperatures between 20 and 37 °C (optimum, 30 °C). Growth was possible at concentrations of 1–10% (w/v) NaCl (optimum, 2%). The phylogenetic trees based on 16S rRNA gene sequences indicated that strain M2BS9Y-3-1 T clustered together with Notoacmeibacter marinus XMTR2A4 T (98.44% similarity) and separated from other species of the family Notoacmeibacteraceae. The average nucleotide identity and in silico DNA–DNA hybridization values between strain M2BS9Y-3-1 T and N. marinus XMTR2A4 T were 76.8%, 19.8%, respectively. The G+C content of the genomic DNA was 60.0 mol%. The cell wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone. The major fatty acid was C 18:1 ω7c. The lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid and three unidentified lipids. The results of taxonomic analysis suggested that strain M2BS9Y-3-1 T represents a novel species of the genus Notoacmeibacter for which the name Notoacmeibacter ruber sp. nov. is proposed. The type strain is M2BS9Y-3-1 T (KCTC 62838 T = CGMCC 1.13746 T ).