The vast majority of the Earth’s biological diversity is hidden in uncultured and yet uncharacterized microbial genomes. The construction of metagenomic libraries is a cultivation-independent molecular approach to assess this unexplored genetic reservoir. High numbers of novel biocatalysts have been identified by function-based or sequence-based screening of metagenomic libraries derived from various environments. Here, we describe detailed protocols for the construction of metagenomic small-insert and large-insert libraries in plasmids and fosmids, respectively, from environmental DNA.
CITATION STYLE
Simon, C., & Daniel, R. (2017). Construction of small-insert and large-insert metagenomic libraries. In Methods in Molecular Biology (Vol. 1539, pp. 1–12). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6691-2_1
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