Molecular and Diagnostic Procedures in Mycoplasmology, Volume II: Diagnostic Procedures

Abstract

This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Front Cover; Molecular and Diagnostic Procedures in Mycoplasmology; Copyright Page; Contents; Contributors; Preface; Contents of Volume I; Mollicute-Host Interrelationships: Current Concepts and Diagnostic Implications; Section A: Diagnostic Genetic Probes; Chapter A1. Introductory Remarks; Chapter A2. Oligonucleotide Probes Complementary to 16S rRNA; Chapter A3. Cloned Genomic DNA Fragments as Probes; Chapter A4. PCR: Selection of Target Sequences; Chapter A5. PCR: Preparation of DNA from Clinical Specimens; Chapter A6. PCR: Amplification and Identification of Products. Chapter A7. PCR: Application of Nested PCR to Detection of MycoplasmasChapter A8. PCR: Random Amplified Polymorphic DNA Fingerprinting; Section B: Immunological Tools; Chapter B1. Introductory Remarks; Chapter B2. ELISA in Small Animal Hosts, Rodents, and Birds; Chapter B3. ELISA in Large Animals; Chapter B4. ELISA in Human Urogenital Infections and AIDS; Chapter B5. ELISA in Respiratory Infections of Humans; Chapter B6. Monoclonal Antibodies as Diagnostic Tools; Chapter B7. Microimmunofluorescence; Chapter B8. Immunoblots and Immunobinding. Chapter B9. Differentiation of Mycoplasma genitalium from Mycoplasma pneumoniae by ImmunofluorescenceSection C: Antibiotic Sensitivity Testing; Chapter C1. Introductory Remarks; Chapter C2. Problems and Opportunities in Susceptibility Testing of Mollicutes; Chapter C3. Determination of Minimal Inhibitory Concentration; Chapter C4. Cidal Activity Testing; Section D: Diagnosis of Specific Diseases; Chapter D1. Introductory Remarks; Chapter D2. Laboratory Diagnosis of Mycoplasma pneumoniae Infection; Chapter D3. Diagnosis of Sexually Transmitted Diseases. Chapter D4. Diagnosis of Neonatal InfectionsChapter D5. Mycoplasmas in AIDS Patients; Chapter D6. Mycoplasma Infections of Cattle; Chapter D7. Mycoplasma Infections of Goats and Sheep; Chapter D8. Mycoplasma Infections of Swine; Chapter D9. Mycoplasma Infections of Poultry; Chapter D10. Diagnosis of Spiroplasma Infections in Plants and Insects; Chapter D11. …