Microbial identification by PCR/electrospray ionization-mass spectrometry

Abstract

Pathogen detection and characterization have traditionally been accomplished through time-consuming, complex and expensive culture-based methods. These methods are reasonably inclusive within the limits of their design, but can only detect inherently cultureable agents that have not been rendered nonviable through preemptive antibiotic treatment, immune system challenge, or other processes. Although culture methods can be used retrospectively to provide an explanation for disease, these assays are often too slow to effectively inform treatment decisions or public health responses. For these reasons, many clinical laboratories have begun to rely on rapid molecular tests such as PCR and rapid antigen strip tests.