The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.
CITATION STYLE
Calarco, J. A., & Friedland, A. E. (2015). Creating genome modifications in C. elegans using the CRISPR/Cas9 system. In Methods in Molecular Biology (Vol. 1327, pp. 59–74). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2842-2_6
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