An HPLC method for microanalysis and pharmacokinetics of marine sulfated polysaccharide PSS-loaded Poly Lactic-co-Glycolic Acid (PLGA) nanoparticles in rat plasma

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Abstract

This study was aimed at developing a sensitive and selective HPLC method with postcolumn fluorescence derivatization for the detection of propylene glycol alginate sodium sulfate (PSS) in rat plasma. Plasma samples were prepared by a simple and fast ultrafiltration method. PSS was extracted from rat plasma with D-glucuronic acid as internal standard. Isocratic chromatographic separation was performed on a TSKgel G2500 PWxL column with the mobile phase of 0.1 M sodium sulfate at a flow rate of 0.5 mL/min. Analyte detection was achieved by fluorescence detection (FLD) at 250 nm (excitation) and 435 nm (emission) using guanidine hydrochloride as postcolumn derivatizing reagent in an alkaline medium at 120°C. The calibration curve was linear over a concentration range of 1-500 μg/mL, and the lower limit of detection (LLOD) was found to be 250 ng/mL. This validated method was applied successfully to the pharmacokinetic study of PSS and PSS-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (PSS-NP) in rat plasma after a single intravenous (PSS only) and oral administration (PSS and PSS-NP). Significant differences in the main pharmacokinetic parameters of PSS and PSS-NP were observed. The relative bioavailability of PSS-NP was 190.10% compared with PSS which shows that PSS-NP can improve oral bioavailability. © 2013 by the authors; licensee MDPI.

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Li, P. L., Li, C. X., Xue, Y. T., Li, H. H., Liu, H. B., He, X. X., … Guan, H. S. (2013). An HPLC method for microanalysis and pharmacokinetics of marine sulfated polysaccharide PSS-loaded Poly Lactic-co-Glycolic Acid (PLGA) nanoparticles in rat plasma. Marine Drugs, 11(4), 1113–1125. https://doi.org/10.3390/md11041113

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