Palaeoclimatic variation, adaptation and biogeography of inversion polymorphisms in natural populations of Drosophila robusta

Abstract

Studies of natural and sexual selection in wild populations of Drosophila have historically provided strong inference for the maintenance of inversion polymorphism. Analysis of geographical variation in the Drosophila robusta chromosomal data collected over more than 50 years from 133 natural populations across eastern North America has confirmed several north-south and east-west clines in the frequencies of some gene arrangements and linked arrangement combinations. Patterns of geographical variation, including several north-south clines, revealed by regression and spatial autocorrelation analyses are concordant with palaeoclimatic shifts, Pleistocene glaciations and historical changes in the composition of North American forest communities. Because D. robusta is a sapbreeder, using the microbe-infested sap exudates of a number of deciduous tree species in which they carry out their life cycle, shifts in climate and palaeovegetation types since the formation of the eastern deciduous forests in the Miocene are hypothesized to be major factors influencing patterns of inversion polymorphisms across the range of this drosophilid species. In areas where sharp deviations in frequencies have been observed, particularly in the midwestern and western portions of the range, these divisions parallel historical geographical disjunctions in the species range that have yet to promote divergence and species formation despite the long history of D. robusta in North America. © 2004 The Linnean Society of London.