Clinical, biological, and immunophenotypical characteristics of B‐cell chronic lymphocytic leukemia with trisomy 12 by fluorescence in situ hybridization

Abstract

The clinical, biological, and immunophenotypical characteristics of B‐cell chronic lymphocytic leukemia (B‐CLL) patients with trisomy 12 detected by fluorescence in situ hybridization (FISH) using a chromosome 12 alpha‐centromeric probe (D12Z3) were analyzed in the present study. From a total of 104 consecutive B‐CLL patients, 21 (20%) displayed trisomy 12, the percentage of trisomic cells ranging from 13% to 76%. From the clinico‐biological point of view, patients with trisomy 12 were associated with atypical CLL morphology (43% vs. 10%, P = 0.04) and BM diffuse pattern (75% vs. 25%, P = 0.02) together with increased WBC counts (141 ± 220 vs. 58 ± 67 × 109/L, P = 0.04). In contrast, no association was detected between the presence of trisomy 12 and other disease characteristics such as age, sex, clinical stage, hepatomegaly, lymphadenopathies, haemoglobin levels and platelet counts, and the cell cycle distribution of PB leukocytes in both groups of patients. Trisomy 12 patients had a significantly higher expression of the FMC7 antigen both in percentage (34 ± 34% vs. 13 ± 20%, P = 0.02) and absolute numbers (29 ± 62 vs. 7 ± 17 × 109/L, P = 0.007). No major differences were found regarding the expression of mouse rosettes, CD19+, and CD19+ /CD5 + lymphocytes. Upon analyzing the correlations between the disease characteristics of trisomy 12 cases, significant associations were found between the percentage of trisomic cells and both the WBC count (r = 0.52, P = 0.02) and the PB lymphocyte count (r = 0.60, P = 0.007). In summary, our results show that although trisomy 12 in B‐CLL is not associated with the clinical stage, it correlates with several prognostic factors and other disease characteristics such as an atypical lymphocyte morphology BM diffuse pattern, high WBC counts, and FMC7 expression. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.