Virus-induced gene silencing: Methods and protocols

Abstract

This book provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. A series of methods is described to tackle the sometimes transient and variable phenotypes for researchers working in diverse fi elds interested in developmental biology, plant–pathogen or herbivore interactions, and plant secondary metabolites. Recently developed protocols for VIGS-derived microRNA production in the plant or protein overexpression are also included in this book, and some chapters are devoted to shortly summarize the molecular mechanisms of VIGS action and the vector systems developed so far and on how to change a plant virus into a VIGS vector.