The modulation of c-di-GMP levels plays a vital role in the regulation of various processes in a wide array of bacterial species. Thus, investigation of c-di-GMP regulation requires reliable methods for the assessment of c-di-GMP levels and turnover. Reversed-phase high-performance liquid chromatography (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from Pseudomonas aeruginosa samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.
CITATION STYLE
Petrova, O. E., & Sauer, K. (2017). High-performance liquid chromatography (HPLC)-based detection and quantitation of cellular c-di-GMP. In Methods in Molecular Biology (Vol. 1657, pp. 33–43). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7240-1_4
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