In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.
CITATION STYLE
McDowell, G., Body, R., Kirwan, C., Byrne, G., & Slevin, M. (2015). A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood. In Handbook of Vascular Biology Techniques (pp. 453–463). Springer Netherlands. https://doi.org/10.1007/978-94-017-9716-0_35
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