Application of stable isotopes to examine N proportions within a simulated Aegiceras corniculatum wetland

Abstract

Salinity levels and drought status of coastal wetlands may be strongly affected by climate change, and changes in the nitrogen cycle of mangrove wetlands may also be affected. We established combinations of three salinity and water levels with applied stable isotope 15N to study the δ15N distributions in the sediment and plants of a greenhouse-based simulated mangrove Aegiceras corniculatum wetland system. The stable isotope 13C and 15N, C and N contents and the C:N ratio were determined. Results showed that increasing in salinity significantly increased the δ13C value in plant organs. The δ15N value of plant organs increased with increasing water level in low salinity (10‰) and medium salinity (20‰) treatment groups but not in the high salinity (30‰) treatment group. This may attributed to A. corniculatum adjusting the δ15N distribution in different organs in response to high salinity stress. Compared to the δ13C, the δ15N values of plant were strongly affected by salinity and water level treatments, indicating that the behavior of N cycle was somewhat different than the C cycle, and affected by the combined effects of both salinity and water level. Most of 15N absorbed by plant tissues were in leaves except for the highest salinity and high water level treatment, showing at increasing water level, the proportion of 15N increased in root. Overall, the measured indicators exhibited different responses to salinity level and water level, suggesting that the changes in salinity and water levels have an impact on N cycling processes of wetland systems.