Antibacterial, antifungal and cytotoxic activities of salviasperanol isolated from Amorphophallus campanulatus

Abstract

Salviasperanol, a diterpenoid isolated from Amorphophallus campanulatus (Roxb.) Bl. (Araceae) was studied for in vitro antibacterial, antifungal, and cytotoxic activities. The disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Cytotoxicity was determined against brine shrimp nauplii. In addition, minimal inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The MIC values against these bacteria ranged from 8 to 64 μg/mL. The compound showed significant antibacterial activity against four Gram-positive bacteria (Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Streptococcus β-haemolyticus) and six Gram-negative bacteria (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonus aeruginosa, Salmonella typhi). The compound showed weak antifungal activity against a number of fungi. In the cytotoxicity determination, the LC50 of the compound against brine shrimp nauplii was 8.02 μg/mL. © 2009 Informa UK Ltd.