Induction of Glyoxylate Cycle-Key Enzymes, Malate Synthase, and Isocitrate Lyase in Ethanol-grown Euglena gracilis

Abstract

The effects of ethanol on the glyoxylate cycle-key enzymes of Euglena, malate synthase (EC 4.1.3.2) and isocitrate lyase (EC 4.1.3.1), were investigated. The addition of ethanol to Euglena cells increased malate synthase and isocitrate lyase activities. Immunoblot analysis with antibody raised against malate synthase showed that the increase in malate synthase activity was due to newly synthesized protein. The experimental results reported here demonstrate that ethanol is assimilated by the glyoxylate cycle including the alcohol metabolizing enzymes in Euglena. © 1994, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.