Influence of Rapeseed Protein Hydrolysis Conditions on Animal Cell Growth in Serum-free Media Supplemented with Hydrolysates

Abstract

Different protein hydrolysates have been prepared from enzymatic hydrolyses of a rapeseed isolate (> 90% protein content) using commercial enzymes of non-animal origin. The extent of hydrolysis has been controlled to prepare various hydrolysates corresponding to different hydrolysis degree (DH) from 5 to 30. The results showed CHO promoting-growth effects of several hydrolysates especially those obtained at high hydrolysis degree. However, different hydrolysates exhibiting same peptide size patterns and similar amino acids compositions did not improve the cell growth in the same way.\r\rNo free online copy. No paper copy in library. Should request. (It's a book).