Background: Existing methods for directly measuring photosynthetic capacity (A max) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchange systems are not designed to maximize throughput, on either a cost or time basis. Results: We present a novel multiplexed semi-portable gas exchange system, OCTOflux, that can measure A max with approximately 4-7 times the throughput of commercial devices, despite a lower capital cost. The main time efficiency arises from having eight leaves simultaneously acclimate to saturating CO2 and high light levels; the long acclimation periods for each leaf (13.8min on average in this study) thus overlap to a large degree, rather than occurring sequentially. The cost efficiency arises partly from custom-building the system and thus avoiding commercial costs like distribution, marketing and profit, and partly from optimizing the system's design for A max throughput rather than flexibility for other types of measurements. Conclusion: Throughput for A max measurements can be increased greatly, on both a cost and time basis, by multiplexing gas streams from several leaf chambers connected to a single gas analyzer. This can help overcome the bottleneck in breeding and ecological research posed by limited phenotyping throughput for A max.
CITATION STYLE
Salter, W. T., Gilbert, M. E., & Buckley, T. N. (2018). A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements. Plant Methods, 14(1). https://doi.org/10.1186/s13007-018-0347-y
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