The polymorphic adp-ribosyltransferase (nad+) pseudogene 1 in humans interrupts an endogenous pol-like element on 13q34

Abstract

A recently constructed chromosome 13-enriched library was used to isolate the entire human ADP-ribosyltransferase (NAD4) pseudogene 1 gene (ADPRTP1) on 13q34. Recently, ADPRTP1 was shown to detect a restriction fragment length polymorphism that was associated with a predisposition to cancer. The complete sequence of the ADPRTP1 (3808 bases) was determined and found to resemble an intronless cDNA, including 137 bases of the 5′ untranslated region and a short poly(A) tail. A previously uncharacterized, endogenous pol-like element (1.53 kb) in which the DNA sequences were interrupted by the polymorphic ADPRTP1 was identified. The solitary pol -related retroviral element may represent a multimember family of viral-like DNA sequences dispersed throughout the human genome. Altogether a 9.25-kb genomic region that also encompassed two Alu elements and the long-terminal repeat-like element of the “O”-LTR family was characterized. © 1993 Academic Press, Inc.