Enhancement of antibody production by addition of ubiquinone-Q10

Abstract

The productivity has a directly influence to the cost of manufacturing for therapeutic monoclonal antibodies (Mab). We have screened enhancer of specific Mab production rate (SPR) using YB2/0 cell-line. We found that coenzyme Q 10 (Q 10) as a media ingredient seems to have no significant influence on the cumulative viable cell density, but has the potential to enhance SPR. We added SANOMIT-Q 10 (MSE, Hamburg, Germany) to medium, we call SANOMIT-Q 10 containing medium as Q-media, at several concentrations and conducted fed-batch culture. Although Q10 is well known strong anti-oxidant, 8-hydroxy-2'-deoxyguanosine (8-OHdG) was no effect by Q-media and Q Lactate / Q Glucose (L/G) was slightly decreased. We evaluate application of Q-media with YB2/0, Chinese Hamster Ovary (CHO), NS0 cell lines. Q-Media enhanced SPR significantly: Case YB2/0: 66.3 %, Case CHO: 28.8%, Case NS0: 31.9 %. On the other hand, the product characteristics have not been affected. For this reason, Q 10 seems to be a powerful ingredient to enhance of biotechnology processing.