Computational protein design, a process that searches for mutants with desired improved properties, plays a central role in the conception of many synthetic biology devices including biosensors, bioproduction, or regulation circuits. To that end, a rational workflow for computational protein design is described here consisting of (a) searching in the sequence, structure or chemical spaces for the desired function and associated protein templates; (b) finding the list of potential hot regions to mutate in the parent proteins; and (c) performing in silico screening of mutants with predicted improved properties.
CITATION STYLE
Carbonell, P., & Trosset, J. Y. (2015). Computational protein design methods for synthetic biology. Methods in Molecular Biology, 1244, 3–21. https://doi.org/10.1007/978-1-4939-1878-2_1
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