Steroid receptors are members of a subfamily of the nuclear receptor superfamily. They play a dual role of steroid hormone receptors and transcription factors. Actually, these receptors are steroid-activated transcription factors. Classical soluble receptors exist as oligomeric complexes with the Hsp90-based chaperone machinery. The steroid receptor field was born and developed along with the molecular chaperone field. Chaperones are not exclusive partners associated to these receptors, but also comprise a large variety of heterocomplexes with other proteins involved in signal transduction. By using the glucocorticoid receptor (GR) as a standard model for most Hsp90-client proteins, in this chapter we describe the functional GR·Hsp90 heterocomplex assembly system from reticulocyte lysate or purified proteins.
CITATION STYLE
Mazaira, G. I., & Galigniana, M. D. (2019). Reconstitution of the steroid receptor heterocomplex. In Methods in Molecular Biology (Vol. 1966, pp. 125–135). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9195-2_10
Mendeley helps you to discover research relevant for your work.